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synthesis 80532-66-7

як ADB-PINACA cooking (2024-01-19)

In the context of next-generation sequencing (NGS), "synthesis sequence" refers to the process of DNA sequencing by synthesis. This method is widely used in NGS platforms, such as Illumina sequencing, to determine the order of nucleotides in a DNA molecule.

Here's a brief overview of the synthesis sequence process in NGS:

  1. Library Preparation: The DNA sample is fragmented into smaller pieces and adapters are added to the ends of the fragments. These adapters contain sequences that allow the DNA fragments to bind to a solid support, such as a flow cell.

  2. Bridge Amplification: The DNA fragments are attached to the flow cell and undergo bridge amplification. This process involves the creation of clusters of DNA fragments, where each cluster contains multiple copies of the same DNA fragment.

  3. Sequencing by Synthesis: The synthesis sequence step begins with the addition of fluorescently labeled nucleotides to the flow cell. These nucleotides are complementary to the template DNA strand and are added one at a time. As each nucleotide is incorporated into the growing DNA strand, its fluorescence is detected.

  4. Imaging and Cleavage: After each nucleotide incorporation, the flow cell is imaged to capture the fluorescent signal. The fluorescence is then cleaved, removing the fluorescent label and blocking further nucleotide incorporation.

  5. Repeated Cycles: The process of nucleotide incorporation, imaging, and cleavage is repeated multiple times to sequence the entire DNA fragment. Each cycle adds a single nucleotide to the growing DNA strand, allowing the determination of the DNA sequence.

By analyzing the fluorescent signals obtained during the synthesis sequence process, the order of nucleotides in the DNA fragment can be determined. This information is then used to reconstruct the original DNA sequence.